private void FileNo_txt_Leave(object sender, System.EventArgs e)
{
//check if that file number is exist in the database or not.
}
also add this line in your constructor(form Constructor)
this.FileNo_txt.Leave+= new System.EventHandler(this.FileNo_txt_Leave);
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solved How to execute code when I move from textbox to the next one in c#? [closed]