Sounds like there are two parts to this question:
1. Getting an image from a video
I suppose you manage to do this since you did not provide any info on the video file format.
2. Detecting a QR code in an image.
Multiple options exist:
-
Use the AForgeTry using AForge.NET library for capturing the video from your webcam, and then ZXing.Net library for reading the QR codes: see QR code webcam scanner c#
-
See Decode QR-Code in WPF By Zxing
1
solved C# – Upload video and read QR codes in it